Resource:BiSearch: Primer Design and Search Tool

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Resource:BiSearch: Primer Design and Search Tool

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Name:	Resource:BiSearch: Primer Design and Search Tool
Description:	BiSearch is a primer-design algorithm for DNA sequences. It may be used for both bisulfite converted as well as for original not modified sequences. You can search various genomes with the designed primers to avoid non-specific PCR products by our fast ePCR method. This is especially recommended when primers are designed to amplify the highly redundant bisulfite treated sequences. It has the unique property of analyzing the primer pairs for mispriming sites on the bisulfite-treated genome and determines potential non-specific amplification products with a new search algorithm. The options of primer-design and analysis for mispriming sites can be used sequentially or separately, both on bisulfite-treated and untreated sequences. In silico and in vitro tests of the software suggest that new PCR strategies may increase the efficiency of the amplification.
Parent Organization:	Hungarian Academy of Sciences; Budapest; Hungary
Supporting Agency:	PXE International Inc., Boolyai Janos Scholarship
Resource Type(s):	Data analysis service
Keywords:	DNA, Sequence, Primer, Design, Algorithm, Analysis, PRiming, Bisulfite, Genome, Amplification, In vitro, In silico, Amplification, ePCR, cytosines
Grant:	GVOP-3.1.1-2004-05-0143/3.0, OTKA T34131, D42207
Abbreviation:	BiSearch
Resource:	Resource
URL:	http://bisearch.enzim.hu
PMID:	PMID 17022803, 15653630
Id:	nif-0000-30170
Link to OWL / RDF:	Download this content as OWL/RDF

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Facts about Resource:BiSearch: Primer Design and Search ToolRDF feed

Abbrev	BiSearch +
CurationStatus	curated +
DefiningCitation	http://bisearch.enzim.hu  +
Definition	BiSearch is a primer-design algorithm for … BiSearch is a primer-design algorithm for DNA sequences. It may be used for both bisulfite converted as well as for original not modified sequences. You can search various genomes with the designed primers to avoid non-specific PCR products by our fast ePCR method. This is especially recommended when primers are designed to amplify the highly redundant bisulfite treated sequences. It has the unique property of analyzing the primer pairs for mispriming sites on the bisulfite-treated genome and determines potential non-specific amplification products with a new search algorithm. The options of primer-design and analysis for mispriming sites can be used sequentially or separately, both on bisulfite-treated and untreated sequences. In silico and in vitro tests of the software suggest that new PCR strategies may increase the efficiency of the amplification. rease the efficiency of the amplification.
Grant	Category:GVOP-3.1.1-2004-05-0143/3.0 +, Category:OTKA T34131 +, and Category:D42207 +
Has default formThis property is a special property in this wiki.	Resource +
Has role	Data analysis service +
Id	nif-0000-30170 +
Is part of	Hungarian Academy of Sciences; Budapest; Hungary +
Keywords	DNA +, Sequence +, Primer +, Design +, Algorithm +, Analysis +, PRiming +, Bisulfite +, Genome +, Amplification +, In vitro +, In silico +, EPCR +, and Cytosines +
Label	Resource:BiSearch: Primer Design and Search Tool +
ModifiedDate	6 September 2011 +
PMID	17022803, 15653630 +
Page has default formThis property is a special property in this wiki.	Resource +
SuperCategory	Resource +
Supporting Agency	PXE International Inc. +, and Boolyai Janos Scholarship +