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Resource:BiSearch: Primer Design and Search Tool

Name: Resource:BiSearch: Primer Design and Search Tool
Description: BiSearch is a primer-design algorithm for DNA sequences. It may be used for both bisulfite converted as well as for original not modified sequences. You can search various genomes with the designed primers to avoid non-specific PCR products by our fast ePCR method. This is especially recommended when primers are designed to amplify the highly redundant bisulfite treated sequences.


It has the unique property of analyzing the primer pairs for mispriming sites on the bisulfite-treated genome and determines potential non-specific amplification products with a new search algorithm. The options of primer-design and analysis for mispriming sites can be used sequentially or separately, both on bisulfite-treated and untreated sequences. In silico and in vitro tests of the software suggest that new PCR strategies may increase the efficiency of the amplification.
Abbreviation: BiSearch
Parent Organization: Hungarian Academy of Sciences; Budapest; Hungary
Supporting Agency: PXE International Inc., Boolyai Janos Scholarship
Grant: GVOP-3.1.1-2004-05-0143/3.0, OTKA T34131, D42207
Resource Type(s): Data analysis service
Resource: Resource
URL: http://bisearch.enzim.hu
Id: nif-0000-30170
PMID: PMID 17022803, 15653630
Keywords: DNA, Sequence, Primer, Design, Algorithm, Analysis, PRiming, Bisulfite, Genome, Amplification, In vitro, In silico, Amplification, ePCR, cytosines
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Facts about Resource:BiSearch: Primer Design and Search ToolRDF feed
AbbrevBiSearch  +
CurationStatuscurated  +
DefiningCitationhttp://bisearch.enzim.hu  +
DefinitionBiSearch is a primer-design algorithm for BiSearch is a primer-design algorithm for DNA sequences. It may be used for both bisulfite converted as well as for original not modified sequences. You can search various genomes with the designed primers to avoid non-specific PCR products by our fast ePCR method. This is especially recommended when primers are designed to amplify the highly redundant bisulfite treated sequences.


It has the unique property of analyzing the primer pairs for mispriming sites on the bisulfite-treated genome and determines potential non-specific amplification products with a new search algorithm. The options of primer-design and analysis for mispriming sites can be used sequentially or separately, both on bisulfite-treated and untreated sequences. In silico and in vitro tests of the software suggest that new PCR strategies may increase the efficiency of the amplification.
rease the efficiency of the amplification.
GrantCategory:GVOP-3.1.1-2004-05-0143/3.0   +, Category:OTKA T34131   +, and Category:D42207   +
Has default formThis property is a special property in this wiki.Resource  +
Has roleData analysis service  +
Idnif-0000-30170  +
Is part ofHungarian Academy of Sciences; Budapest; Hungary  +
KeywordsDNA  +, Sequence  +, Primer  +, Design  +, Algorithm  +, Analysis  +, PRiming  +, Bisulfite  +, Genome  +, Amplification  +, In vitro  +, In silico  +, EPCR  +, and Cytosines  +
LabelResource:BiSearch: Primer Design and Search Tool  +
ModifiedDate6 September 2011  +
PMID17022803, 15653630  +
Page has default formThis property is a special property in this wiki.Resource  +
SuperCategoryResource  +
Supporting AgencyPXE International Inc.  +, and Boolyai Janos Scholarship  +