arrow

From NeuroLex

Jump to: navigation, search



Resource:CS2 Vector Resource

Name: Resource:CS2 Vector Resource
Description: This web site has been created to provide a centralized source for CS2 and related vector information. It will be updated (on an irregular basis) with additional information, including new vector designs.

CS2 vector: pCS2 is a multipurpose expression vector. Although originally designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2 is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA), and it can be used for in vitro transcription/translation (using, for example, the Promega TnT system). A number of derivatives of CS2 have been constructed that allow fusions to epitope tags and other marker proteins, as well as nuclear localization signals or the gal4 DNA binding and activation domains. In almost all cases, the same reading frames are used for the fusion vectors, to facilitate moving genes between multiple CS2 derivatives. pCS2 features: pCS2 contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS and includes the amp resistance gene and an f1 origin for producing single stranded DNA.

Sponsors: This resource is supported by the University of Michigan.
Keywords: Vector, Resource, Expression, Protein, Xenopus, Embryo, RNA, DNA, Transient, Zebrafish, Domain, mRNA, Synthesis, Promoter,
Other Name(s): CS2 Vector Resource
Parent Organization: University of Michigan; Michigan; USA
Resource Type(s): Reagent supplier
Resource: Resource
URL: http://sitemaker.umich.edu/dlturner.vectors
*Id: nif-0000-30277
Link to OWL / RDF: Download this content as OWL/RDF

Curation status: Uncurated

This resource will be curated within 7 days.

For Resource Owners:
After the resource is curated, you may create a sitemap, which will help keep your registry description up-to-date and inform search engines about your resource.

Note: For a new resource, the website's URL must first be verified by a NIF curator before you may proceed.

Learn more about what NIF can do for your resource.
Proudly proclaim your inclusion in NIF by displaying the "Registered with NIF" button on your site. Please login to create the sitemap. (top right)

This page uses this default form:Resource

Contributors

Ccdbuser, Nifbot2



bookmark

*Note: Neurolex imports many terms and their ids from existing community ontologies, e.g., the Gene Ontology. Neurolex, however, is a dynamic site and any content beyond the identifier should not be presumed to reflect the content or views of the source ontology. Users should consult with the authoritative source for each ontology for current information.

Facts about Resource:CS2 Vector ResourceRDF feed
CurationStatusCurated  +
DefiningCitationhttp://sitemaker.umich.edu/dlturner.vectors  +
DefinitionThis web site has been created to provide This web site has been created to provide a centralized source for CS2 and related vector information. It will be updated (on an irregular basis) with additional information, including new vector designs.

CS2 vector: pCS2 is a multipurpose expression vector. Although originally designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2 is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA), and it can be used for in vitro transcription/translation (using, for example, the Promega TnT system). A number of derivatives of CS2 have been constructed that allow fusions to epitope tags and other marker proteins, as well as nuclear localization signals or the gal4 DNA binding and activation domains. In almost all cases, the same reading frames are used for the fusion vectors, to facilitate moving genes between multiple CS2 derivatives. pCS2 features: pCS2 contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS and includes the amp resistance gene and an f1 origin for producing single stranded DNA.

Sponsors: This resource is supported by the University of Michigan.
Keywords: Vector, Resource, Expression, Protein, Xenopus, Embryo, RNA, DNA, Transient, Zebrafish, Domain, mRNA, Synthesis, Promoter,
rafish, Domain, mRNA, Synthesis, Promoter,
Has default formThis property is a special property in this wiki.Resource  +
Has roleReagent supplier  +
Idnif-0000-30277  +
Is part ofUniversity of Michigan; Michigan; USA  +
LabelResource:CS2 Vector Resource  +
ModifiedDate10 July 2013  +
Page has default formThis property is a special property in this wiki.Resource  +
SuperCategoryResource  +
SynonymCS2 Vector Resource  +