arrow

From NeuroLex

Jump to: navigation, search



Resource:Human Gene and Protein Database (HGPD)

Name: Resource:Human Gene and Protein Database (HGPD)
Description:

The Human Gene and Protein Database presents SDS-PAGE patterns and other informations of human genes and proteins.
The HGPD was constructed from full-length cDNAs. For conversion to Gateway entry clones, we first determined an open reading frame (ORF) region in each cDNA meeting the criteria. Those ORF regions were PCR-amplified utilizing selected resource cDNAs as templates. All the details of the construction and utilization of entry clones will be published elsewhere. Amino acid and nucleotide sequences of an ORF for each cDNA and sequence differences of Gateway entry clones from source cDNAs are presented in the "GW: Gateway Summary" window. Utilizing those clones with a very efficient cell-free protein synthesis system featuring wheat germ, we have produced a large number of human proteins in vitro. Expressed proteins were detected in almost all cases. Proteins in both total and supernatant fractions are shown in the "PE: Protein Expression" window. In addition, we have also successfully expressed proteins in HeLa cells and determined subcellular localizations of human proteins. These biological data are presented on the frame of cDNA clusters in the Human Gene and Protein Database. To build the basic frame of HGPD, sequences of FLJ full-length cDNAs and others deposited in public databases (Human ESTs, RefSeq, Ensembl, MGC, etc.) are assembled onto the genome sequences (NCBI Build 35 (UCSC hg17)).

The majority of analysis data for cDNA sequences in HGPD are shared with the FLJ Human cDNA Database (http://flj.hinv.jp/) constructed as a human cDNA sequence analysis database focusing on mRNA varieties caused by variations in transcription start site (TSS) and splicing.
Other Name(s): HGPD
Parent Organization: National Institute of Advanced Industrial Science and Technology
Resource Type(s): Database
Keywords: gene, cdna clusters, cdnas, cdna sequences, human, in vitro, mrna varieties, protein, sds-page
Resource: Resource
URL: http://www.HGPD.jp
Id: nif-0000-02956
Link to OWL / RDF: Download this content as OWL/RDF

Curation status: Uncurated

This resource will be curated within 7 days.

For Resource Owners:
After the resource is curated, you may create a sitemap, which will help keep your registry description up-to-date and inform search engines about your resource.

Note: For a new resource, the website's URL must first be verified by a NIF curator before you may proceed.

Learn more about what NIF can do for your resource.
Proudly proclaim your inclusion in NIF by displaying the "Registered with NIF" button on your site. Please login to create the sitemap. (top right)

This page uses this default form:Resource

Contributors

Aarnaud, Ccdbuser, Nifbot2



bookmark
Facts about Resource:Human Gene and Protein Database (HGPD)RDF feed
CurationStatuscurated  +
DefiningCitationhttp://www.HGPD.jp  +
Definition

The Human Gene and Protein Databas
The Human Gene and Protein Database presents SDS-PAGE patterns and other informations of human genes and proteins.
The HGPD was constructed from full-length cDNAs. For conversion to Gateway entry clones, we first determined an open reading frame (ORF) region in each cDNA meeting the criteria. Those ORF regions were PCR-amplified utilizing selected resource cDNAs as templates. All the details of the construction and utilization of entry clones will be published elsewhere. Amino acid and nucleotide sequences of an ORF for each cDNA and sequence differences of Gateway entry clones from source cDNAs are presented in the "GW: Gateway Summary" window. Utilizing those clones with a very efficient cell-free protein synthesis system featuring wheat germ, we have produced a large number of human proteins in vitro. Expressed proteins were detected in almost all cases. Proteins in both total and supernatant fractions are shown in the "PE: Protein Expression" window. In addition, we have also successfully expressed proteins in HeLa cells and determined subcellular localizations of human proteins. These biological data are presented on the frame of cDNA clusters in the Human Gene and Protein Database. To build the basic frame of HGPD, sequences of FLJ full-length cDNAs and others deposited in public databases (Human ESTs, RefSeq, Ensembl, MGC, etc.) are assembled onto the genome sequences (NCBI Build 35 (UCSC hg17)).

The majority of analysis data for cDNA sequences in HGPD are shared with the FLJ Human cDNA Database (http://flj.hinv.jp/) constructed as a human cDNA sequence analysis database focusing on mRNA varieties caused by variations in transcription start site (TSS) and splicing.
ption start site (TSS) and splicing.
Has default formThis property is a special property in this wiki.Resource  +
Has roleDatabase  +
Idnif-0000-02956  +
Is part ofNational Institute of Advanced Industrial Science and Technology  +
KeywordsGene  +, Cdna clusters  +, Cdnas  +, Cdna sequences  +, Human  +, In vitro  +, Mrna varieties  +, Protein  +, and Sds-page  +
LabelResource:Human Gene and Protein Database (HGPD)  +
ModifiedDate20 June 2013  +
Page has default formThis property is a special property in this wiki.Resource  +
SuperCategoryResource  +
SynonymHGPD  +