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Resource:PrimerBank

Name: Resource:PrimerBank
Description: Database of human and mouse primer pairs for gene expression analysis by polymerase chain reaction (PCR) and quantitative PCR (qPCR).

A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.

All experimental validation data for mouse primers are available from PrimerBank.

You can submit your primers. They will be added to the database once they are properly QCd.[1]
Other Name(s): PrimerBank: PCR Primers for Gene Expression Detection and Quantification
Abbreviation: PrimerBank
Parent Organization: Harvard Medical School; Massachusetts; USA
Supporting Agency: NIH
Grant: U01 HL66678
Resource Type(s): Database, Data repository
Resource: Resource
URL: http://pga.mgh.harvard.edu/primerbank/
Id: nif-0000-21333
PMID: PMID 22086960, 19906719, 19108745, 14654707
Publication link: http://nar.oxfordjournals.org/content/38/suppl_1/D792.full
Related to: Resource:OMICtools
Availability: Public, Acknowledgement requested, The community can contribute to this resource
Address: Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114-2790, USA.
Keywords: electrophoresis, gene expression, Quantitative PCR, gel, gene, agarose, algorithm, amplification, human, molecular probe, primer database, mouse, PCR, primer, primer pair, protein, quantification, reaction, secondary structure, Polymerase Chain Reaction, Real-time PCR, PCR Primer, Detection, BLAST
Organism: Human, Mouse
Link to OWL / RDF: Download this content as OWL/RDF

Curation status: Curated

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References

  1. Spandidos A et al. (2010) PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 38: D792-9 PubMed

Notes

This page uses this default form:Resource

Xref: OMICS_02323

Contributors

Aarnaud, Ccdbuser, Nifbot2



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Facts about Resource:PrimerBankRDF feed
AbbrevPrimerBank  +
AddressDepartment of Genetics  +, Harvard Medical School  +, Center for Computational and Integrative Biology  +, Massachusetts General Hospital  +, Boston  +, MA 02114-2790  +, and USA.  +
AvailabilityPublic  +, Acknowledgement requested  +, and The community can contribute to this resource  +
CurationStatuscurated  +
DefiningCitationhttp://pga.mgh.harvard.edu/primerbank/  +
DefinitionDatabase of human and mouse primer pairs f Database of human and mouse primer pairs for gene expression analysis by polymerase chain reaction (PCR) and quantitative PCR (qPCR).

A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.

All experimental validation data for mouse primers are available from PrimerBank.

You can submit your primers. They will be added to the database once they are properly QCd.
o the database once they are properly QCd.
ExampleImagePrimerBank.PNG  +
GrantCategory:U01 HL66678   +
Has default formThis property is a special property in this wiki.Resource  +
Has roleDatabase  +, and Data repository  +
Idnif-0000-21333  +
Is part ofHarvard Medical School; Massachusetts; USA  +
KeywordsElectrophoresis  +, Gene expression  +, Quantitative PCR  +, Gel  +, Gene  +, Agarose  +, Algorithm  +, Amplification  +, Human  +, Molecular probe  +, Primer database  +, Mouse  +, PCR  +, Primer  +, Primer pair  +, Protein  +, Quantification  +, Reaction  +, Secondary structure  +, Polymerase Chain Reaction  +, Real-time PCR  +, PCR Primer  +, Detection  +, and BLAST  +
LabelResource:PrimerBank  +
ModifiedDate30 May 2014  +
PMID22086960, 19906719, 19108745, 14654707  +
Page has default formThis property is a special property in this wiki.Resource  +
PublicationLinkhttp://nar.oxfordjournals.org/content/38/suppl_1/D792.full  +
RelatedToResource:OMICtools  +
SpeciesHuman  +, and Mouse  +
SuperCategoryResource  +
Supporting AgencyNIH  +
SynonymPrimerBank: PCR Primers for Gene Expression Detection and Quantification  +