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Resource:PrimerBank

Name: Resource:PrimerBank
Description: Public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR).

A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.

There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene Symbol, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DB.

The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency.They have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis.

All experimental validation data for mouse primers are available from PrimerBank.

You can submit your primers. They will be added to the database once they are properly QCd.[1]
Other Name(s): PrimerBank: PCR Primers for Gene Expression Detection and Quantification
Parent Organization: Harvard Medical School; Massachusetts; USA
Supporting Agency: NIH
Resource Type(s): Database, Data repository
Keywords: electrophoresis, gene expression, Quantitative PCR, gel, gene, agarose, algorithm, amplification, human, molecular probe, primer database, mouse, PCR, primer, primer pair, protein, quantification, reaction, secondary structure, Polymerase Chain Reaction, Real-time PCR, PCR Primer, Detection
Grant: U01 HL66678
Abbreviation: PrimerBank
Resource: Resource
URL: http://pga.mgh.harvard.edu/primerbank/
PMID: PMID 19906719, 19108745, 14654707
Publication link: http://nar.oxfordjournals.org/content/38/suppl_1/D792.full
Availability: Public, The community can contribute to this resource., Please cite the papers describing the work done for the PrimerBank database. PrimerBank IDs should also accompany the cited primer pairs.
Address: Department of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114-2790, USA.
Id: nif-0000-21333
Organism: Human, Mouse
Link to OWL / RDF: Download this content as OWL/RDF

Curation status: Uncurated

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References

  1. Spandidos A et al. (2010) PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 38: D792-9 PubMed

Notes

This page uses this default form:Resource

Contributors

Aarnaud, Ccdbuser, Nifbot2



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Facts about Resource:PrimerBankRDF feed
AbbrevPrimerBank  +
AddressDepartment of Genetics  +, Harvard Medical School  +, Center for Computational and Integrative Biology  +, Massachusetts General Hospital  +, Boston  +, MA 02114-2790  +, and USA.  +
AvailabilityPublic  +, The community can contribute to this resource.  +, and Please cite the papers describing the work done for the PrimerBank database. PrimerBank IDs should also accompany the cited primer pairs.  +
CurationStatuscurated  +
DefiningCitationhttp://pga.mgh.harvard.edu/primerbank/  +
DefinitionPublic resource for the retrieval of human Public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR).

A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.

There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene Symbol, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DB.

The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency.They have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis.

All experimental validation data for mouse primers are available from PrimerBank.

You can submit your primers. They will be added to the database once they are properly QCd.
o the database once they are properly QCd.
ExampleImagePrimerBank.PNG  +
GrantCategory:U01 HL66678   +
Has default formThis property is a special property in this wiki.Resource  +
Has roleDatabase  +, and Data repository  +
Idnif-0000-21333  +
Is part ofHarvard Medical School; Massachusetts; USA  +
KeywordsElectrophoresis  +, Gene expression  +, Quantitative PCR  +, Gel  +, Gene  +, Agarose  +, Algorithm  +, Amplification  +, Human  +, Molecular probe  +, Primer database  +, Mouse  +, PCR  +, Primer  +, Primer pair  +, Protein  +, Quantification  +, Reaction  +, Secondary structure  +, Polymerase Chain Reaction  +, Real-time PCR  +, PCR Primer  +, and Detection  +
LabelResource:PrimerBank  +
ModifiedDate8 May 2013  +
PMID19906719, 19108745, 14654707  +
Page has default formThis property is a special property in this wiki.Resource  +
PublicationLinkhttp://nar.oxfordjournals.org/content/38/suppl_1/D792.full  +
SpeciesHuman  +, and Mouse  +
SuperCategoryResource  +
Supporting AgencyNIH  +
SynonymPrimerBank: PCR Primers for Gene Expression Detection and Quantification  +