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Pages using the property "ChemoDefiningCriteria"

Showing 20 pages using this property.

A

Area 29 of isthmus of DMVH2003 +Calbindin D28K staining clearly differenti Calbindin D28K staining clearly differentiates area 29 from the presubiculum of the isthmus. The granular layer of area 29i is totally devoid of CB+ neuropil, in contast to the presubiculum part, which is darkly labeled for neuropil in the deep part (layer 3) of the granular cell layer. Parvalbumin staining is observed all layers with the exception of layer 1 in 29i, compared to the more intense labeling for PV in the granular layer of the presubiculum compared to other layers. the presubiculum compared to other layers.
Area prostriata of DMVH2003 +Slightly higher density of parvalbumin+ ce Slightly higher density of parvalbumin+ cells and neuropil in the anterior portion compared to the adjacent parasubiculum, but lower compared to area 29i and to the posterior portion of the parastriate area. The difference between the parastriate area and area 18 is most clearly defined in SMI-32 stained sections, with few SMI-32+ cells in the Pro compared to area 18. -32+ cells in the Pro compared to area 18.

B

Bed nucleus of the stria medullaris +Stained intensely for enkephalin mRNA usin Stained intensely for enkephalin mRNA using in situ hybridization compared to the bed nucleus of the anterior commissure, but less intensely than in the lateral septal nucleus and bed nuclei of the stria terminalis. Caudal to the BSM, enkephalinergic neurons gradually became more scattered and the nucleus disappeared at the rostral end of the diencephalon, in particular, at what appears to be the rostral border of the reticular nucleus of the thalamus. In contrast, cell bodies expressing GAD 67 mRNA were very sparse, contrasting sharply with very abundant labeling observed in the lateral septal nucleus and bed nuclei of the stria terminalis, and to a lesser extent in the septofimbrial nucleus. More caudally, in the rostral pole of the reticular nucleus of the thalamus perikarya expressing GAD 67 mRNA were very abundant. Immunolabeling for enkephalin was seen in perkarya after colchicine injection. en in perkarya after colchicine injection.

C

CA1 of RHA11 +In material stained for parvalbumin or ACh In material stained for parvalbumin or AChE, the pyramidal layer of CA1 is darkly stained, whereas the pyramidal cell layer of the subiculum is more diffusely stained, thus indicating a marked border between the two fields. In the TIMM staining the border is also visible, but then with an unstained CA1pyramidal layer, and a darkly stained cell layer in the proximal part of the subiculum. Although the border may appear easy to establish, it is in practice not possible to determine whether a particular dendrite at the border belongs to a neurone in CA1 or in the subiculum. This is caused by the oblique orientation of the CA1 / subiculum border relative to the transverse axis. Therefore, cells (in the stratum oriens, stratum radiatum, or lacunosum moleculare) close to this border do not necessary extent their dendrites perpendicularly to the orientation of stratum pyramidale. to the orientation of stratum pyramidale.

D

Dentate gyrus of RHA11 +With a stain for calbindin, the hilus is c With a stain for calbindin, the hilus is clearly set apart from the adjacent proximal part of CA3 as a positive area, similar to the stained mossy fibers that emanate from DG reaching cells in the hilus as well as providing the main connection to CA3. This staining pattern is mimicked by staining with an antibody against dynorphin. aining with an antibody against dynorphin.

E

Entorhinal cortex of RHA11 +The medial border with the parasubiculum f The medial border with the parasubiculum features: decreased staining intensity and homogeneity for calbindin in the neuropil of all layers; dense staining for acetylcholinesterase in layers I-III in parasubiculum. The lateral and caudal borders between EC and the perirhinal and postrhinal cortices are distinguished by parvalbumin and calbindin staining. The part of EC at the border stains densely for parvalbumin whereas the adjacent perirhinal and postrhinal cortex are almost devoid of positive staining; the latter areas stain for calbindin which is much less conspicuously present in the adjacent parts of EC. Layer II of EC is characterized by a population of large to medium sized neurons that stain very densely for neuronal markers such as Nissl or NeuN. The adjacent parts of perirhinal and postrhinal cortices are characterized by blended layers II and III consisting of small, lightly stained neurons The anterior border of EC is indicated by: weak overall staining for parvalbumin in both anterior parts of EC as well as the neighboring areas, so this does not provide a clear indication of the border; staining for calbindin that shows less dense staining in the anterior portions of EC compared to the adjacent regions ons of EC compared to the adjacent regions

N

Nucleus paramedianus dorsalis +It contains calretinin immunoreactive larg It contains calretinin immunoreactive large cells with oval or polygonal cell bodies. Cells are not immunoreactive for either calbindin or parvalbumin, but a few fibers immunoreactive to each protein are found within its central region. Cells in PMD are also immunoreactive to nNOS, and immunoreactivity to a neurofilament protein shows many labeled cells and fibers. The nucleus is much more prominent in sections processed for calretinin immunoreactivity compared to Nissl-stained sections. tivity compared to Nissl-stained sections.

O

Ongur, Price, and Ferry (2003) area 10m +cholinesterase: Moderately stained plexus in layers I/II, III, and V immunocytochemistry - SMI32: Many stained cells; darkly stained neurite plexus immunocytochemistry - parvalbumin: Many darkly staining cells in layer V; fewer in layer III
Ongur, Price, and Ferry (2003) area 10r +cholinesterase: Moderately stained plexus cholinesterase: Moderately stained plexus in layers I/II, III, and V immunocytochemistry - SMI32: Many stained cells; darkly stained neurite plexus immunocytochemistry - parvalbumin: Moderate numbers of stained cells in layers III and V; double-banded appearance layers III and V; double-banded appearance
Ongur, Price, and Ferry (2003) area Ial +cholinesterase: Moderate plexus in layers II-VI SMI32 immunoreactivity: Many stained cells (++) Parvalbumin immunoreactivity: Band of stained neurons in layer V++

P

Paleocortex +Three to five cytoarchitectural layers
Parabigeminal nucleus +Majority of cells are cholinergic. ChAT-positive cell bodies of the parabigeminal nucleus are oval in shape and 10-20/um in diameter, as measured in mouse.
Parasubiculum of the isthmus of DMVH2003 +Distinguished from the adjacent area OA (area 19) and presubiculum by lighter parvalbumin staining.
Perirhinal cortex of rodent of Burwell et al 1995 +Stains darkly for Timm's sulphide silver t Stains darkly for Timm's sulphide silver technique compared to entorhinal cortex, although it provides only a partial indication of the dorsal border of area 36 (Fig 5). Entorhinal cortex stains darkly for parvalbumin compared to perirhinal cortex (Fig. 5). in compared to perirhinal cortex (Fig. 5).
Postrhinal cortex of rodent of Burwell et al 1995 +Stains intensely with Timm's method and li Stains intensely with Timm's method and lightly for actetylcholinesterase. Caudomedially, the postrhinal cortex is bounded by a thin strip of parasubiculum lying inbetween the postrhinal and entorhinal cortices. The parasubiculum stains intensely for acetylcholinesterase. stains intensely for acetylcholinesterase.
Postsubiculum +Cell islands in layer 2 do not stain for A Cell islands in layer 2 do not stain for AChE, in contrast to the area surrounding the islands tha is densely stained. In Vogt silver stained material, the post subiculum is characterized by a dense fiber plexus in layer 1a and extremely few fibers in layer II in corast to the even distribution of stained fibers in layer 1-III in the presubiculum. Layer 1 is evenly stained compared to a patches of light and dark staining in the retrosplenial granular a cortex. Layer III is stained in postsubiculum but not in the retrosplenial granular a cortex. ot in the retrosplenial granular a cortex.
Presubiculum of isthmus of DMVH2003 +In calbindin D-28K stained sections, PrSi In calbindin D-28K stained sections, PrSi is clearly distinguished from 29i by the dark staining of neuropil in layer III (deep part) of the granular cell layer. In contrast, the granular cell layer of 29i is completely devoid of calbindin + neuropil. PrSi stains intensely for parvalbumin in the granular layer (cell bodies and processes) although other layers have lighter staining. In contrast, area29i shows dark staining for parvalbumin in all layers except layer 1. parvalbumin in all layers except layer 1.

R

Red nucleus +Complement component 1, q subcomponent-like 2 (C1QL2) marker for red nucleus neurons.

S

Serotonergic cell group B8 +Cells stain for serotonin
Subiculum of RHA11 +At the border between CA1 and the subiculu At the border between CA1 and the subiculum, there is an abrupt change in the staining for calbindin in that the positive cells and rather intense stained neuropil of stratum pyramidale of CA1, and also the staining in stratum lacunosum-moleculare, is replaced by the almost complete negative corresponding layer in Sub. This change coincides with a change in the appearance of the superficial fiber layer. In CA1, one can rather easily differentiate between stratum radiatum, characterized by the aligned orientation of apical dendrites and the more superficial stratum lacunosum-moleculare. This laminar separation is absent in Sub. In sections stained for parvalbumin, the pyramidal layer of CA1 is darkly stained, whereas the pyramidal cell layer of the subiculum is more diffusely, but still rather densely stained, thus indicating a marked border between the two fields. In material stained for parvalbumin or AChE, the pyramidal layer of CA1 is darkly stained, whereas the pyramidal cell layer of the subiculum is more diffusely stained, thus indicating a marked border between the two fields. ng a marked border between the two fields.

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