arrow

From NeuroLex

Jump to: navigation, search
Category:Resource:Trinity Biobank
Abbrev Trinity Biobank  +
Address Trinity Biobank  +, Institute of Molecular Medicine  +, Trinity Centre for Health Sciences  +, St James's Hospital  +, Dublin 8  +, Ireland  +
Comment Sample type: DNA, Blood, Tissue, Buffy coat, Cell, Packed cell, Cultured cell, Buccal cell, Whole blood, Bodily fluid
CurationStatus curated  +
DefiningCitation http://www.tcd.ie/IMM/trinity-biobank/index.php  +
Definition The Trinity Biobank was established in 200 The Trinity Biobank was established in 2005 to serve the needs of researchers in the area of genetic epidemiology, population genetics and pharmacogenomics. Its services are available to researchers not only in Trinity College but to other institutions at home and abroad. We provide an automated DNA extraction service purifying large volumes blood (up to 10mL whole blood) and tissue DNA for archival and other purposes. In addition it makes available purified DNA and associated GWAS data from 2000 healthy donors for research use. <br /> A key requirement for reliable downstream use of DNA is purity and strand size. The quality of DNA in blood and tissue deteriorates upon storage without purification even at -80 degrees C. We ensure rapid turnaround of biological samples through automated extraction using the Qiagen Autopure system based on optimized 'salting out' chemistry. The purified DNA sample may then be stored safely at -20 degrees C without deterioration thus freeing up valuable -80 degree C freezer space and the associated capital and maintenance cost as well as security and lab space provision. Automated DNA extraction is particularly suitable for high-throughput sample processing called for in epidemiological studies or simply for clearing sample inventory backlogs. The Trinity Biobank distributes control DNA to researchers as part of its remit to enhance the level of research activity and to synergize molecular medicine research nationally and internationally. The buffy coat collection has been made possible with the cooperation of the Irish Blood Transfusion Service (IBTS). An important requirement to access the collection is that the use of the samples relates only to ethically-approved research and to specifically-nominated research projects. The DNA collection consists of high quality human genomic DNA. Each of the available 2,000 samples is from a single individual and each sample comes with the age and gender data of the donor. The buffy coat sample is derived from the total white cell compliment (50mL buffy coat) of a blood donation (c 400mL). We will endeavor to fulfill samples number requests based on age and gender as best as possible. This collection has also been genotyped using the Affymetrix Genome-Wide Human SNP Array 6.0, featuring 1.8 million genetic markers, including more than 906,600 single nucleotide polymorphisms (SNPs) and more than 946,000 probes for the detection of copy number variation (CNV). The DNA comes available as a 100ng/uL in 100uL of TE Buffer, ie in 10ug amounts in a separate screw-cap ampoule. The ampoules are shipped in 100-tube boxes (Sarstedt). Corresponding plasma (ACD) is also available on request. Genotype data is supplied in PLINK binary PED files format (http://pngu.mgh.harvard.edu/~purcell/plink/ ). ://pngu.mgh.harvard.edu/~purcell/plink/ ).
ExampleImage Trinity Biobank.PNG +
Has default formThis property is a special property in this wiki. Resource  +
Has improper value forThis property is a special property in this wiki. Availability  +
Has role Biomaterial supply resource +, Material service resource +, Material storage repository +, Data set +
Id nlx_25068  +
Is part of Trinity College Dublin; Dublin; Ireland +, St. James Hospital; Dublin; Ireland +
Keywords DNA +, Blood +, Tissue +, Buffy coat +, Cell +, Packed cell +, Cultured cell +, Buccal cell +, Whole blood +, Bodily fluid +, Fresh +, Frozen +, Normal +, Normal +, Genetic epidemiology +, Population genetics +, Pharmacogenomics +, Gene +, Genetics +, Genome-wide association study +, Genome-wide association study data +, Genotype +, DNA extraction +, Non-mammalian +, Bacteria +, Gram-positive bacteria +, Gram-negative bacteria +
Label Resource:Trinity Biobank  +
Modification dateThis property is a special property in this wiki. 27 July 2012 01:50:12  +
ModifiedDate 27 July 2012  +
Page has default formThis property is a special property in this wiki. Resource  +
Processing Fresh +, Frozen +
RelatedTo Resource:One Mind Biospecimen Bank Listing +, European Biobank +
Related application Research  +
Related disease Normal +, Normal +
Species Human +
SuperCategory Resource  +
Categories Resource
hide properties that link here 
  No properties link to this page.
 

 

Enter the name of the page to start browsing from.