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Abbrev PrimerBank  +
Address Department of Genetics  +, Harvard Medical School  +, Center for Computational and Integrative Biology  +, Massachusetts General Hospital  +, Boston  +, MA 02114-2790  +, USA.  +
Availability Public +, Acknowledgement requested +, The community can contribute to this resource +
CurationStatus curated  +
DefiningCitation  +
Definition Database of human and mouse primer pairs f Database of human and mouse primer pairs for gene expression analysis by polymerase chain reaction (PCR) and quantitative PCR (qPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.<br /> All experimental validation data for mouse primers are available from PrimerBank. You can submit your primers. They will be added to the database once they are properly QCd. o the database once they are properly QCd.
ExampleImage PrimerBank.PNG +
Grant Category:U01 HL66678   +
Has default formThis property is a special property in this wiki. Resource  +
Has role Database +, Data repository +
Id nif-0000-21333  +
Is part of Harvard Medical School; Massachusetts; USA +
Keywords Electrophoresis +, Gene expression +, Quantitative PCR +, Gel +, Gene +, Agarose +, Algorithm +, Amplification +, Human +, Molecular probe +, Primer database +, Mouse +, PCR +, Primer +, Primer pair +, Protein +, Quantification +, Reaction +, Secondary structure +, Polymerase Chain Reaction +, Real-time PCR +, PCR Primer +, Detection +, BLAST +
Label Resource:PrimerBank  +
Listedby Resource:OMICtools +
Modification dateThis property is a special property in this wiki. 12 March 2015 23:15:56  +
ModifiedDate 12 March 2015  +
PMID 22086960, 19906719, 19108745, 14654707  +
Page has default formThis property is a special property in this wiki. Resource  +
PublicationLink  +
Species Human +, Mouse +
SuperCategory Resource  +
Supporting Agency National Institutes of Health +
Synonym PrimerBank: PCR Primers for Gene Expression Detection and Quantification  +
Xref OMICS_02323  +
Categories Resource
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*Note: Neurolex imports many terms and their ids from existing community ontologies, e.g., the Gene Ontology. Neurolex, however, is a dynamic site and any content beyond the identifier should not be presumed to reflect the content or views of the source ontology. Users should consult with the authoritative source for each ontology for current information.