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Category:Resource:PrimerBank
Abbrev PrimerBank  +
Address Department of Genetics  +, Harvard Medical School  +, Center for Computational and Integrative Biology  +, Massachusetts General Hospital  +, Boston  +, MA 02114-2790  +, USA.  +
Availability Public +, Please cite the papers describing the work done for the PrimerBank database. PrimerBank IDs should also accompany the cited primer pairs. +, The community can contribute to this resource +
CurationStatus curated  +
DefiningCitation http://pga.mgh.harvard.edu/primerbank/  +
Definition Public resource for the retrieval of human Public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. All primers in PrimerBank were carefully designed to ensure gene specificity.<br /> There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene Symbol, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DB. The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency.They have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis. All experimental validation data for mouse primers are available from PrimerBank. You can submit your primers. They will be added to the database once they are properly QCd. o the database once they are properly QCd.
ExampleImage PrimerBank.PNG +
Grant Category:U01 HL66678   +
Has default formThis property is a special property in this wiki. Resource  +
Has role Database +, Data repository +
Id nif-0000-21333  +
Is part of Harvard Medical School; Massachusetts; USA +
Keywords Electrophoresis +, Gene expression +, Quantitative PCR +, Gel +, Gene +, Agarose +, Algorithm +, Amplification +, Human +, Molecular probe +, Primer database +, Mouse +, PCR +, Primer +, Primer pair +, Protein +, Quantification +, Reaction +, Secondary structure +, Polymerase Chain Reaction +, Real-time PCR +, PCR Primer +, Detection +
Label Resource:PrimerBank  +
Modification dateThis property is a special property in this wiki. 28 June 2013 18:55:36  +
ModifiedDate 28 June 2013  +
PMID 19906719, 19108745, 14654707  +
Page has default formThis property is a special property in this wiki. Resource  +
PublicationLink http://nar.oxfordjournals.org/content/38/suppl_1/D792.full  +
Species Human +, Mouse +
SuperCategory Resource  +
Supporting Agency National Institutes of Health +
Synonym PrimerBank: PCR Primers for Gene Expression Detection and Quantification  +
Categories Resource
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